Thesis

The Effect of Freezing Time Variation In Freeze-Drying on Mesenchymal Stem Cells (MSCs)-Secretome Potency Invitro on 3T3 Cells

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Secretome is a bioactive substance secreted by MSCs in a conditioned media, which consists of
growth factors, other substances that are able to provide a biological response by undergoing signal
transduction. A secretome-based therapy has gained attention as a novel cosmetic active ingredient
for its antioxidant and wound healing properties. To overcome the stability issue, freeze-drying
technique was used to preserve secretome from degradation, into a convenient, ready-made
preparation for subsequent formulation, storage, and transport. As the freezing step is the first critical
step in the freeze-drying, ice crystal morphology is affecting the quality of the end product. This
research was aimed to observe the effect of the freezing time parameter toward the secretome
protein stability which can be evaluated from the secretome potency on the 3T3 fibroblast cell,
through the oxidative stress assay and in-vitro scratch assay. Prior to the potency evaluations, total
protein content quantification and cytocompatibility test were done to determine the secretome
concentrations and the safe concentrations of secretome, resveratrol, and H
2
O
toward the cells,
respectively. From the oxidative stress assay and in-vitro scratch assay, the FD secretome from 24
hours of freezing time was found to have the highest cytoprotective properties against H
and
induce the fastest wound healing activity. Meanwhile, the non-FD secretome was found to have no
cytoprotective and cell proliferation and migration influence toward the fibroblast cells. Therefore, it
can be concluded that freeze-drying with sufficient freezing time is crucial in preserving secretome
from instability.

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Availability

Detail Information

Series Title

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Call Number

PHA 23-003

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

freeze-drying
Secretome
Umbilical cord-Mesenchymal stem cell (UC-MSC)
Oxidative stress assay
In-vitro scratch assay

Specific Detail Info

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Statement of Responsibility

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