Thesis

The Effect of Freezing Time Variation in Freeze-Drying on Protein Characteristics of Mesenchymal Stem Cell-Secretome (MSC-S)

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esenchymal stem cells (MSCs) have been long studied and developed as a regenerative medicine
supported by their secreted bioactive molecules called secretome or MSC-secretome (MSC-S) that
contain soluble factors and extracellular vesicles (EVs). Unlike the MSC-based therapy that utilized the
whole cells, secretome is a cell-free therapy that would ease the future treatment development as it
would not have a rejection reaction to the recipient and the result is similar to the MSC-based
therapy. In its native form isolated as a liquid formulation, secretome might have a short shelf life as it
cannot withstand the aqueous condition for a long period, especially the proteins that can be
denatured thereby degrading its effect as a therapeutic agent. To overcome this, a freeze-drying or
lyophilization method was performed as a means to maintain protein stability and increase the shelf
life. The freezing time as one of the critical process parameters (CPPs) in the freeze-drying process
varied for 24, 12, and 6 hours and the process was carried out with the addition of 8% (v/v) trehalose
as the cryoprotectant for secretome. Evaluation on the protein characteristics of lyosecretome
showed that the freezing time for 24 hours was able to retain the highest total protein content and a
little higher phospholipid concentration although not significant, compared to the 12 and 6 hours.
The SDS-PAGE result showed no significant differences from all freezing times but protein degradation
was clearly observed when compared to the fresh secretome.

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Availability

Detail Information

Series Title

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Call Number

PHA 23-010

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

freeze-drying
Freezing time
MSC secretome
lyosecretome
protein characteristics

Specific Detail Info

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Statement of Responsibility

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