Thesis

The Role of Receptor Activity Modifying Proteins (RAMPs) in Pancreatic Cancer

---

Pancreatic Cancer (PaCa) is the 5th leading cause of cancer-related death in the UK. Understanding
the signalling behind PaCa development is essential to create novel treatment for PaCa. RAMPs are
family of single transmembrane domain proteins. The interaction between RAMP and GPCR give rise
to AM receptor complex. AM is a hormone that has been shown to promote progression of various
tumours including PaCa. This study was conducted to understand the role of RAMPs family in PaCa.
The result showed that RAMPs were expressed at mRNA and protein levels in two PaCa cell lines,
BxPC-3 and CFPAC-1. The optimization of seeding densities, FCS concentration, and transfection
conditions were established prior to conducting RAMPs knockdown using siRNA. The efficacy of
siRNA-mediated knockdown at mRNA and protein level were validated using qPCR and western blot,
respectively. Subsequently, the effect of knockdown towards PaCa metabolic activity and apoptosis
were assessed. Results showed knockdown of RAMPs did not exert significant effect on PaCa cell
metabolic activity (p=0.1). However, it did affect PaCa cell apoptosis, as evidenced by remarkable
increase in Caspase 3/7 level by 450% and 570% for knockdown RAMP1 and 2, respectively. Hence, it
indicates the role of RAMP1 and 2 are important in maintaining cancer survival by reducing their
apoptosis rate.

---

Availability

Detail Information

Series Title

-

Call Number

BM 23-010

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

-

Language

English

ISBN/ISSN

-

Classification

NONE

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

Receptor-Activity Modifying Proteins (RAMPs)
Adrenomedullin (AM)
Pancreatic Cancer (PaCa)

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment