Thesis

The Evaluaon of An-aging Properes of Marchana paleacea Extracts Against ROS-induced Damage in HaCaT Cells Through Gene Expression Analysis on MMP1 and COX2

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Aging is a universal phenomenon which affects all living organisms; a gradual degradation of form
and function as the body succumbs to ever accumulating adverse changes. The skin is the largest and
most superficial organ of the human body; as such it is exposed to external stressors which would
increase the production of ROS, the primary actor of the aging process. As a response, the cell will
activate stress-related signaling cascades which would cause changes in the expression level of genes
such as MMP1 and COX2; genes that contribute to the aging skin phenotype through collagen
degradation and triggering cellular senescence, respectively. Antioxidants are commonly used to
slow down the aging process as they inhibit the adverse effects of ROS. However there is a need to
switch towards natural antioxidants due to the unsustainable nature and the potential adverse
effects of synthetic antioxidants. Marchantia paleacea is a bryophyte common to Indonesia that
possesses natural antioxidant capabilities, however the exact molecular mechanism of its anti-aging
properties is still unclear. To investigate, cytotoxic and antioxidant assays, along with a cytoprotective
study, as well as a gene expression analysis was performed on HaCaT cells under ROS-induced
oxidative stress to assess whether or not Marchantia paleacea extracts would alter the expression
levels of MMP1 and COX2. M. paleacea extracts were found to possess cytotoxic activity against
HaCaT cells. It was also discovered that the extract were capable of scavenging DPPH
(2,2-diphenyl-1-picrylhydrazyl) radicals as well as protecting the HaCaT cells against H
oxidative damage, hinting at its role as a versatile antioxidant. The extract however was not able to
significantly influence the expression levels of MMP1 and COX2, as such the extract could not be
considered as an anti-aging agent through the alteration in the expression levels of the two genes.

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Availability

Detail Information

Series Title

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Call Number

BM 23-009

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Aging
ROS
Marchantia paleacea
antioxidants
gene expression analysis
MMP1
COX2

Specific Detail Info

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Statement of Responsibility

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