Thesis

An in vitro study of Pogonatum neesii antioxidant properties towards HaCaT cells

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Reactive Oxygen Species (ROS) is a natural byproduct that plays many important roles for the body such
as cell signaling, cell survival, and cell death. However, the accumulation of ROS can cause damage to
the cell that leads to cell death and eventually to aging. To overcome that, various studies look for
antioxidant substances, such as phenolic, flavonoids, and alkaloids. As the study of natural-based
compounds as antioxidants becomes more important because of its safer characteristics, Pogonatum
neesii might be a good candidate for the study due to the previous study which was shown to have no
cytotoxic effects towards HaCaT cells. Since skin is generally one of the main organs exposed to the
external factor, HaCaT has been widely used to study antioxidants activities. Hence, this study will
investigate the radical scavenging abilities and the antioxidant properties of Pogonatum neesii under
oxidative stress in oxidative stress-triggered HaCaT cells through gene expression analysis using GPX1
and SOD2 which are widely known to have radical neutralizing abilities. To examine and determine the
best concentration of Pogonatum neesii extract for gene expression analysis, an MTS assay was
performed and 50 ppm was determined to be the most suitable concentration for gene expression
analysis. Pogonatum neesii has been shown to have a high phenolics and alkaloid content through
quantitative screening and has a radical scavenging ability which has been tested using DPPH assay (IC
= 174.5 ppm). The gene expression analysis shows that Pogonatum neesii extract is only able to increase
GPX1 expression but not SOD2. Thus, showing the potential of Pogonatum neesii as an antioxidant.

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Availability

Detail Information

Series Title

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Call Number

BM 23-006

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Pogonatum neesii
antioxidants
Reactive Oxygen Species
HaCaT cell

Specific Detail Info

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Statement of Responsibility

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