Thesis

Prediction of T-Cell and B-Cell Epitopes From Protein Allergen Tropomysin That are Presented by Major HLA Alleles in The Indonesian Population

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hellfish allergy is one of the highest prevalence allergies in the world, accounting for 7.7% of the
population including Indonesia. Thus, the development of T cell epitope and B cell epitope based
vaccines play an important role in future prospects for alternative treatment for allergy. The vaccine
aims to induce the immune system so it will not react towards the allergen though the body already
went through sensitization. In this experiment, immunoinformatics methods were used to predict the
T cell and B cell epitopes from Pen a 1 allergen, tropomyosin from Penaeus aztecus. T cell epitopes
were obtained from NetCTLpan for HLA Class I binding peptide and NetMHCIIpan for HLA Class II
binding peptide. As for B cell epitopes, three different servers that are complementary to each other
were used; BepiPred 2.0, IEDB B Cell Epitope Prediction Tool, and ABCpred. T cell epitopes were
analyzed further for their immunogenicity, IFN-gamma-inducing ability, cross-reactivity against
human peptides, and conservancy in other tropomyosin sequences. The vaccine was then
constructed using promiscuous T cell and B cell epitopes using beta-defensin as adjuvant and
appropriate linkers. The vaccine construct has good population coverage and therefore potential to
be developed as an allergen immunotherapy for the Indonesian population

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Availability

Detail Information

Series Title

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Call Number

BM 23-004

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

immunoinformatics
shellfish allergy
multi epitope peptide-based vaccine
pen a 1
cytotoxic T cells
helper T cells

Specific Detail Info

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Statement of Responsibility

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