Proceedings

The Screening of Natural Autophagy-Inducing Compounds for Anti-Intracellular Bacteria Activity in Keratinocytes

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With the increasing concern about finding a different cure to overcome bacterial skin infections, this research aims to investigate the ability of the innate cell ability to decrease intracellular bacteria infection through autophagy. This study explores and decides several potential naturally-derived autophagy-inducing compounds to be applied to Escherichia coli-infected keratinocytes (HaCaT cells). Those compounds are screened through a double-fluorescent proteins-tagged system called GFP-RFP-LC3. Both green and red fluorescent proteins are tagged to LC3 protein, an essential protein that regulates the accomplishment of autophagic flux inside the cells. The observation of autophagosomes and autolysosomes is done using high-content imaging and they appear as puncta which are visualized by GFP and RFP. Those number of puncta are then quantified to determine the autophagic activity. To sum up, of the various compounds tested, E133 and S1157 have been selected as the most effective autophagy-inducing natural compounds for autophagy-mediated bacterial reduction testing, and those compounds successfully decrease the intracellular bacterial quantity to around 59 and 57 percent respectively. Compared to cells under normal conditions that are fully infected (100%) by the bacteria.

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Availability

Detail Information

Series Title

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Call Number

BM 23-021

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Escherichia coli
HaCaT cells
Autophagy
Autophagosome
Autolysosome
GFP-RFP-LC3

Specific Detail Info

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Statement of Responsibility

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