Proceedings

Anti-Aging Properties: The study of aging-related Genes on HaCat Cell Line Under Bryophytes (Pogonatum neesii) Extract Treatment

---

Skin is the largest human organ which functions as the first barrier, which gradually ages over time and under stressful circumstances from the presence of ROS and finally results in skin disorders. Where the increased expression of the MMP-1 and COX-2 genes is one of the indicators of aging. Thus, a source of anti-aging is required. Natural sources with anti-aging characteristics are currently prevalent and this study used P.neesii, a member of a class of bryophytes that has received minimal investigation. Through this study, the potential anti-aging properties of P.neesii were examined through phytochemical screening, cell viability assay, and qRT PCR utilizing HaCat cells as a model. This study demonstrates that P.neesii extract in 80% methanol has alkaloids, flavonoids, and significant levels of phenol tannins and has a radical scavenging activity of antioxidants. Moreover, it is non-toxic and may protect cells from H₂O₂ exposure at certain concentrations. In addition, the geneexpression analysis revealed that P.neesii treatment did not significantly reduce the expression of MMP-1 yet further confirmation is advisable due to nonspecific binding. Moreover, the COX-2 study in gene expression revealed a significant downregulation of its gene under mixed P.neesii treatment.

---

Availability

Detail Information

Series Title

-

Call Number

BM 23-019

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

-

Language

English

ISBN/ISSN

-

Classification

NONE

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

Anti-Aging
Pogonatum neesii
MMP-1
COX-2

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment