Proceedings

Restoration of X-ray Irradiation-Induced Intestinal Damage by Gut Microbiota

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The gut microbiota determines the intestinal health status of the host by regulating gut epithelial and immune functions. Depletion of the beneficial gut microbiota after radiation exposure contributes to an exaggerated inflammatory response and dysregulation of intestinal epithelial cells. Previous studies demonstrated that administration of beneficial gut microbiota, Lactobacillus acidophilus, could restore damaged gut indicated by the increased intestinal epithelial cell proliferation and differentiation. Preliminary study has discovered two beneficial bacteria isolated from human feces, MHL0043 and MHL0044, corresponding to their anti-inflammatory properties. However, their potential role in mitigating radiation-induced intestinal damage has not been explored. Therefore, the current study aimed to investigate the role of MHL0043 and MHL0044 in the recovery of irradiation-induced intestinal damage. C57BL/6J mice were irradiated at various doses (1, 4 and 7 Gy) to assess the effects of X-ray radiation on intestinal epithelium. Furthermore, HT-29 cells were exposed to X-ray radiation at 15 Gy to mimic the aberrant phenotypes of intestinal epithelium and then co-cultured with the candidate bacteria. Treatment of irradiated cells with MHL0044, but not MHL0043, showed more promising recovery phenotypes as indicated by enhanced intestinal epithelial cell proliferation and differentiation. MHL0044 also rescued the integrity of intestinal epithelium by upregulating the gene expressions of tight junctions. Downregulated expression levels of pro-inflammatory cytokines were also observed upon treatment with MHL0044. Taken together, this study provides the insight into the potential role of MHL0044 for the treatment of irradiation-induced intestinal injury.

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Detail Information

Series Title

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Call Number

BM 23-025

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

gut microbiota
immune homeostasis
intestinal barrier integrity
intestinal epithelial cell
Xray irradiation

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