Proceedings

Study of Itchy Leaf Extract as New Possible Antiviral Properties Against Newcastle Disease Virus

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Laportea decumana often known as itchy leaf, is a Papuan wild plant. Its antiviral activity was investigated for future recommendations of possibly new medication development, and the NDV virus was employed as a safe model for infection. To investigate the toxicity of itching leaves, a cytotoxicity experiment employing MTS was performed. Plaque assays were performed to evaluate the antiviral activities of itchy leaf extract. Finally, qRT-PCR was performed to see if the TLR8 gene was increased after infection and treatment. The qRT-PCR search for gene amplification for GAPDH and TLR8 was unsuccessful since no sigmoid curve was seen. Thus, It is impossible to determine TLR8 gene upregulation. Positive antiviral capabilities were discovered during the experiment following plaque assay, however more study is needed to completely understand how itchy leaf might limit virus propagation.

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Availability

Detail Information

Series Title

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Call Number

BM 23-023

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

qRT-PCR
Antiviral
Itchy leaf
NDV
plaque assay

Specific Detail Info

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Statement of Responsibility

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