Proceedings

Method Development for Investigating SARM1 Post Translational Modification towards Axon Degeneration

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Axon degeneration played a part in the development of neurodegenerative disease for instance Alzheimer’s diseases, chemotherapy-induced neuropathy, and traumatic brain injury. It is found that axon degeneration is present prior to the onset of symptoms. Therefore, scrutinizing the molecular mechanism is detrimental. Recently, it has been found that SARM1 NADase play a role in the developmental of axon degeneration. The activation further cleaves the NAD+ and consequently results in the disintegration of the membrane through the loss of ATP, mitochondrial depolarization, and loss integrity of the membrane. Thus, this research would want to optimize method to validate the post translational modification of SARM1 during the self-inhibited, activation and inhibited state. This experiment successfully optimized the SARM1 activation assay where the Vacor portrayed its toxicity properties towards SHSY5Y cell line activating the SARM1. In addition, we managed to enrich the SARM1, detecting the protein in the digested peptides and recorded a single phosphorylation in the Serine residue on the position 54 that is highly conserved and exposed, showing evidence that indeed a post-translational modification is present and future works of validation and screening is necessary to eventually target the specific changes with kinases and drugs.

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Detail Information

Series Title

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Call Number

BM 23-033

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

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Classification

NONE

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Carrier Type

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Edition

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Subject(s)

SARM1
Post-translational modification
axon degeneration
neurodegenerative diseases

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