Proceedings

Developing Protein Complexes for Targeted Aggregation on Cancer Cell Surfaces via Glycan Recognition

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Cancer is one of the deadliest diseases worldwide, with metastasis serving as the primary cause of death. While metastasis has been widely studied, a significant amount still remains unexplored, particularly the role of glycosylation. Glycosylation is a fundamental aspect in a variety of biological processes, indicating that slight changes in it can lead to detrimental effects such as cancer, where a form of aberrant glycosylation (sialylation - an upregulation of sialic acids) is the hallmark of cancer cells, making them a suitable therapeutic target. However, normal mammalian cells also possess sialic acids, and hence a more specific therapy to target cancer cells is what is desired. Therefore, this study involves production of protein-complexes consisting of a Bacterial Microcompartment (BMC) shell protein, PduU from the Pdu BMC, attached to a lectin (galectin-3) as a possible therapeutic option for cancer cells. These complexes were produced by artificially expressing them in BL21 (DE3) E. coli cells through transformation, followed by the growth of selected colonies and an overexpression of the protein of interest. Then, FPLC was conducted to purify the protein, followed by an analysis of the purification through SDS-PAGE, after which dialysis was performed to concentrate the protein of interest, which finally underwent lyophilization. The FPLC chromatograms obtained showed peaks representing the presence of proteins, while the SDS-PAGE gels resulted in bands corresponding to the molecular weights of the protein of interest. Finally, lyophilization led to the production of a whitish powder, indicating the successful production of the protein complex of interest.

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Availability

Detail Information

Series Title

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Call Number

EP BT029

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Cancer cells
hypersialylation
bacterial microcompartment (BMC)
FPLC
Lyophilization

Specific Detail Info

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Statement of Responsibility

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