Proceedings

Effect of Spray Drying on The Antimicrobial Activity of Different Pediococcus acidilactici Cell Concentrations

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Pediococcus acidilactici has gained a lot of interest lately due to its ability as a potential probiotic strain. However, concerns regarding its viability and stability during consumption and storage lead to a solution to utilize spray dry as an encapsulation method for Pediococcus acidilactici. Yet, the utilization of spray drying could affect the cell viability and its functional probiotic properties due to stress exposure. This study evaluated the effect of spray drying on specifically antimicrobial activity of Pediococcus acidilactici. In addition, different cell concentrations will also be tested in this study to determine their effects on the bacteria’s antimicrobial activity. Through this study, spray drying of different cell concentrations (Log 9, Log 7, Log 6 CFU/ml) of Pediococcus acidilactici was conducted under the same parameters, followed by antimicrobial activity testing with non-spray-dried cells using agar well diffusion assay and time-kill assay against two common foodborne pathogens, Staphylococcus aureus and Enterohemorrhagic Escherichia coli. Initially, the agar well diffusion assay suggested that only non-spray-dried cells with Log 9 CFU/ml exerted a strong antimicrobial effect on both pathogens. However, the time-kill assay indicated that all three cell concentrations showed inhibitory effects on both pathogens, with bactericidal effects shown against S. aureus and bacteriostatic effects against E. coli EHEC. Moreover, the effect of different cell concentrations on antimicrobial activity varies between both pathogens, where it shows no significant difference (p > 0.05) in the reduction of viable S. aureus cells whereas for E. coli EHEC, it shows a significant difference (p < 0.05) specifically the Log 7 CFU/ml which has significantly lower viable pathogens compared to the other two concentrations. Ultimately, it indicated that spray drying showed no significant difference (p > 0.05) in the antimicrobial activity of Pediococcus acidilactici.

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Detail Information

Series Title

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Call Number

BT 22-009

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

BT 22-009

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

spray drying
Antimicrobial Activity
Pediococcus acidilactici
probiotic encapsulation
cell

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