Proceedings

The Effects of BAP and NAA on Callus Regeneration of Vanilla planifolia Andrew. Var. Vania 2

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An expensive Vanilla planifolia Andrew. Var. Vania 2 is commonly propagated using a stem
cutting technique which is associated with low propagation rate. A method of callus regeneration was
assessed in this experiment to increase the yield of vanilla plants by utilizing different concentrations
and combinations of BAP and NAA supplemented into the MS media. The objectives of this
experiment were to propagate the seed production of Vania 2 variety by callus regeneration and to
find the most favored concentrations and combinations of BAP and NAA for callus regeneration. BAP
was used with the concentration of 0, 0.1, 0.5, and 1 mg/L and NAA with the concentration of 0, 0.1,
and 0.5 mg/L. The parameters assessed in this experiment were shoot emergence time, shoots
number, shoots height, roots number, and roots length. All of the experimental data were analysed
with one-way ANOVA using SPSS IBM26. The statistical results indicated that B0.1 + N0.1 showed to
be the fastest in shoot emergence (29 ± 8.18 days). The highest shoots number regenerated was
using B0.5 + N0.1 (14.67 ± 2.51 shoots) and the highest shoots was using B0.1 + N0.1 (5.17 ± 1.78
cm). Furthermore, the highest roots number found using B0.1 + N0.1 (7 ± 1 roots) and the longest
roots found using B0 + N0.5 (12.07 ± 2.31 cm). Depending on each research goals, the best media of
each parameter can be used.

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Availability

Detail Information

Series Title

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Call Number

BT 22-011

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

BT 22-011

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Plant tissue culture
BAP
Vanilla planifolia Andrew
Var. Vania 2
Callus regeneration
NAA

Specific Detail Info

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Statement of Responsibility

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