Proceedings

Effect of Whey Protein and Gum Arabic Ratios on Survivability and Physicochemical Properties of Spray-dried Pediococcus acidilactici

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Pediococcus acidilactici has gained research interest due to its potent probiotic
characteristics. However, it has low survivability in the gastrointestinal tract (GIT). Thus, spray drying
is commonly used to improve its survival. Although current studies obtained high spray drying
encapsulation efficiency, further evaluation of its survival during GIT and viability during storage is
not well-studied. Moreover, the most studied encapsulation material is orange juice, limiting its
application to orange-flavored products. Hence, this study investigated the effect of Whey Protein
(WP) and Gum Arabic (GA) as alternative spray drying encapsulation materials for P. acidilactici on its
encapsulation efficiency, viability during storage, survival during GIT simulation, and physicochemical
properties (production yield, moisture content, water activity, Fourier-transform infrared
spectroscopy (FTIR) profile, and scanning electron microscopy (SEM) analysis). Three formulations of
WP to GA ratios were tested (A=1:1, B=3:1, C=1:3). Samples had 87.13 to 93.08% encapsulation
efficiency, 84.93 to 95.04% survival during GIT simulation, and maintained sufficient viable cell (7 Log
CFU/g) during 3 weeks of storage (25oC). The product yield was 63.12-67.10%, with 4.66-5.91%
moisture content and 0.17-0.21 water activity. The FT-IR showed structural changes in WP and GA,
indicating the physical binding of the encapsulation matrix. Lastly, the SEM result showed
semi-spherical microcapsules in all samples. Significant effects of varying WP to GA ratios were found
in the GIT survival and SEM results. The sample with higher WP content (B) has higher survival and a
smoother microcapsule surface. These findings suggest that WP and GA can be promising
encapsulation materials for P. acidilactici spray drying, especially used at a higher WP to GA ratio.

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Availability

Detail Information

Series Title

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Call Number

BT 22-013

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

BT 22-013

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

spray drying
Encapsulation
Pediococcus acidilactici
probiotic
whey protein
gum arabic

Specific Detail Info

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Statement of Responsibility

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