Proceedings

The Problem Identification in Growing Positive Control From Alicyclobacillus Species Detection Methodology

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PT. Saraswanti Indo Genetech is a laboratory company that provides detection services for
quality control for other companies that need permission from BPOM to commercialize their
product. This project is one of the company’s projects for expanding its scope of services. The
project’s goal is to identify the problem in growing the positive control of the IFU (The International
Fruit and Vegetable Juice Association) method for detecting Alicyclobacillus bacteria. Based on the
personnel in the company, due to the nature of the acidophilic and thermophilic bacteria,
Alicyclobacillus is quite challenging to grow. Therefore, troubleshooting in growing the control
positive is needed because control positive is necessary when detecting the Alicyclobacillus
acidoterrestris. The experiment is first growing the Alicyclobacillus acidoterrestris through
enrichment. To confirm that the bacteria have been successfully grown, a spore stain and peroxidase
test on guaiacol production are performed. After conducting the experiment with several repetitions,
the results indicate that the Alicyclobacillus acidoterrestris has successfully grown and been
detected. Therefore, it can be concluded that there is no problem in growing the positive control of
Alicyclobacillus acidoterrestris.

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Availability

Detail Information

Series Title

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Call Number

BT 22-017

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

BT 22-017

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Quality control
PT. Saraswanti Indo Genetech
Alicyclobacillus

Specific Detail Info

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Statement of Responsibility

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