Proceedings

In-vivo Calcium Activity Profiling Of Mhb Terminals In The Ipn During Fear Memory Acquisition And Retrieval In Mammals

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The medial habenula (MHb) is the less explored subregion of the emerging brain structure, Habenula (Hb) with roles in fear memory expression, anxiety, and addiction. At the neuron terminals where MHb neurons project to, the interpeduncular nucleus (IPN), vesicular neurotransmitter release is triggered by the influx of calcium ions via Ca v2.3 calcium channels, and robustly potentiated by GABAB receptors. Findings in zebrafish had previously shown an asymmetry in the roles of left and right MHb-IPN pathways on the behavior of larval zebrafish. The current project sets out to investigate the possibility of similar asymmetry in mammals using calcium imaging at MHb terminals in the IPN coupled with fear conditioning behavior experiments. Calcium activity tended to decrease during freezing episodes, although more replicates would be needed to verify this. GABAB receptor knockout experiments implied that the right MHb participates in fear attenuation while the left MHb facilitates it, although more circuit studies are needed to support or deny this hypothesis. Research looking into this matter from inside and outside the MHb pathway, with different methods and approaches, is recommended and has a great potential due to the Hb’s role in fear behaviors and fear-related disorders.

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Detail Information

Series Title

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Call Number

EP BM-019

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

EP BM-019

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Edition

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Subject(s)

medial habenula
habenulopeduncular pathway
asymmetry
live calcium imaging
fiber photometry
fear conditioning

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