Thesis

Side Effect Of Vigabatrin As An Anti-epileptic Drug Observed In Mouse Model

---

Infantile spasm is a type of epilepsy that occurs in infants that is characterized by the sudden movement throughout the whole body. It is crucial to detect the disease and undergo suitable treatments as soon as possible in order to minimize the potential negative long-term effect in learning development. One of the most effective treatments is the usage of anti-epileptic drug Vigabatrin that its efficacy has been proven to reduce seizure. However, some recent research showed that there was a neuroinflammation effect caused by vigabatrin consumption. Prolonged neuroinflammation in the central nervous system is heavily correlated with neurodegenerative effect. Therefore, this research was conducted to ensure the side effect of vigabatrin. In this experiment, Luxol fast blue staining and immunofluorescence staining were used to determine the amount of microglia and astrocytes positive cells. Western blot analysis was further used to determine the expression level of proinflammatory proteins. The result showed that vigabatrin group samples had higher astrocytes number, indicating the activation of astrocytes as the first part that induce neuroinflammation effect. The proinflammatory protein level, such as GFAP and Iba1, was shown to have higher expression in the VGB group. Additionally, levels of inflammatory cytokines were also higher in the VGB group, further proving the neuroinflammation occurrence after VGB treatment.

---

Availability

Detail Information

Series Title

-

Call Number

EP BT-009

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

-

Language

English

ISBN/ISSN

-

Classification

EP BT-009

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

Infantile spasm
Vigabatrin
Neuroinflammation
Microglia activation
Reactive astrocytes
Mice brain

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment