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Stards Knockdown Reveals DLC3 Involvement in Vascular Generation and Immune Cell Migration

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The Deleted in Liver Cancer family houses the RhoGAP proteins DLC1 and DLC2, which have already been known for their tumor suppressive capabilities, and DLC3, whose studies regarding this gene are minimal. This study seeks to elucidate the function of DLC3 protein towards tumor development by looking at the changes in angiogenesis and cell migration rate following DLC3 downregulation, using zebrafish as an observation and infection model. The downregulation was achieved by knocking down the STARD8 gene, which encodes for the DLC3 protein, using injections of CRISPR-Cas9 systems into fertilized single-cell zebrafish embryos. M. marinum infection was used to form granuloma in zebrafish, mimicking tumor conditions. Findings showed that STARD8 knockdown, which leads to the downregulation of DLC3 affects cellular functions, such as the infectivity level of bacteria indicated by granuloma formation, angiogenesis, and cell migration indicated by immune cell migration towards the site of inflammation, albeit no significant differences were observed. Yet, this study could be used as a preliminary study for future research working on a similar topic.

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Availability

Detail Information

Series Title

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Call Number

EP BM-018

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

EP BM-018

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

STARD8
DLC3
knockdown
angiogenesis
cell migration
CRISPR-Cas9

Specific Detail Info

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Statement of Responsibility

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