Enrichment Program

Lipid Accumulation From Mixotrophic Cultivation Of Microalgae Using Low-cost Agricultural Byproducts

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Currently, the demand for sustainable platforms to address environmental problem is arising. Biodiesel is an eco-friendly alternative for regular diesel as it has many advantages, and the most important thing is derived from environmental-friendly source, such as microalgae. Several microalgae are known for being able to synthesize lipids and even higher concentration. Newly microalgal strains namely Chlamydomonas spp. Tai-03 and Chlamydomonas spp. Tai-03 has been taken into account in this research to assess the ability of producing lipids in mixotrophic cultivation, on different glucose concentrations (1 g/L, 3 g/L, 5 g/L, and 7 g/L), and later compare the accumulation. Cultivation conditions were maintained in this research and HPLC were conducted to track the glucose consumption towards the cultivation. Following that, the lipid extraction of Day 14 of cultivation was conducted, involving the Folch method to measure the lipid amount. In Chlamydomonas spp. Tai-03, 5 g/L of glucose concentration were found to accumulate lipid the highest (0,26 g), whilst in Chlamydomonas spp. Tai 04, 3 g/L of glucose concentration accumulate lipid the highest (0,21 g). This results suggest that a suitable amount of glucose is needed to enhance the lipid growth, as the optimum glucose concentration might be strain-specific. As preliminary screening, these findings are expected to assist the further assessment of the properties and traits of the newly strains, and contribute to energy switching and world sustainability.

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Availability

Detail Information

Series Title

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Call Number

EP BT-015

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

EP BT-015

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

lipid accumulation
Glucose
Chlamydomonas spp. Tai-03
Chlamydomonas spp. Tai 04
Folch method

Specific Detail Info

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