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Determination Yeast Community Inoculation Use Takakura Methodology On Food Waste Compost

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This study highlights the environmental consequences of landfill buildup while addressing the issue
of insufficient food waste management in Indonesia and the 1.3 billion yearly instances worldwide.
The study focuses on the Takakura Composting Method (TCM), which combines fermentative
microorganisms and locally created materials to provide a sustainable household-scale solution. The
Takakura composting method includes laying materials in baskets, with two different sample
compositions of rice, vegetable, and fruit waste with sample A has the ratio of 1:1:1 and sample B
consists of 1:1:2. The sample was taken every three days and kept in centrifuge tubes with PBS at 4
°C. As a part of an entire research activity, this research focuses on the topic of fungus and yeast
communities. The results showed that temperature changes throughout the 30-day composting
process revealed microbial-driven organic waste breakdown. The pH differences between samples A
and B were attributed to different organic waste ratios, specifically the greater acidity in sample B
due to the higher fruit content. Both samples had pH levels ranging from 4.5 to 7, with no apparent
difference. putative Aspergillus spp. and putative Trichoderma spp. were identified as fungal strains,
whereas budding yeast (putative Saccharomyces cerevisiae) and fission yeast (putative
Schizosaccharomyces) contributed to organic material degradation. This research looks at the
complex interaction of temperature, pH, and microbial diversity in Takakura composting for long-
term waste management. In conclusion, this research found 22 fungal and 9 yeast samples in
Takakura composting, confirming success for samples A and B. T-test results showed no significant
differences, supporting the method's applicability. Future research should optimize sample ratios,
explore microorganisms' roles, and investigate EM4's characteristics.

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Availability

Detail Information

Series Title

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Call Number

EP BT-020

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

EP BT-020

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Fungi
Food waste
Yeast
Microbes
Composting
Takakura

Specific Detail Info

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Statement of Responsibility

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Other version/related

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