Report

Elucidating The Impact of EBV Infection on Cell Cycle Dynamic

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This research aims to analyze the impact of EBV (Epstein-Barr virus) infection on cell cycle phases (G1, S,
G2/M) during latent and lytic phases, exploring alterations in cell cycle regulatory proteins. The
investigation extends to compare fixation methods' ethanol and paraformaldehyde. The focus lies in
comprehending EBV's effect on host cell cycle progression, emphasizing viral reactivation. The study
employs D98 cell lines, selectively targeting EBV gene-containing cells with puromycin. Tamoxifen
stimulation prompts viral reactivation after cell synchronization with RO-3306. Cell cycle analysis,
achieved through propidium iodide (PI) staining using flow cytometry. Expected outcomes anticipate
distinctive cell cycle distributions between latent and lytic phases, especially within G1, S, and G2/M
phases. Additionally, the hypothesis suggests ethanol fixation may yield superior results, considering its
ability to penetrate cells. This research broadens understanding regarding EBV's impact on host cell cycle
regulation during different infection phases. It has implications for elucidating viral replication
mechanisms and potential therapeutic targets

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Availability

Detail Information

Series Title

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Call Number

EP BM-001

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

EP BM-001

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Flow Cytometry
EBV infection
cell cycle phases
tamoxifen stimulation
cell fixation
viral

Specific Detail Info

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Statement of Responsibility

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