Report

Investigation Of Virus-host Interaction Upon Rna Virus Infection

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The overarching goal of this project is to comprehend the intricate dynamics of RNA viruses, specifically influenza A viruses, enteroviruses, and coronaviruses, which pose significant health threats. The focus lies in exploring hostŸvirus interactions, particularly the roles of host components in various stages of viral RNA replication, aiming to identify potential targets for antiviral interventions. The research centres on the interaction between two key proteins¡PA ¥encoded by influenza A virus¦ and host protein P ¥a mitochondrial protein with potential antiviral properties¦¡with the hypothesis that PA contributes to viral replication by binding to Host Protein P. Major findings involve the confirmation of this interaction through plasmid transfection, immunoprecipitation, and Western blot techniques. However, challenges such as nonspecific FLAG signals were acknowledged. Further investigations include assessing Host Protein P°s antiviral properties and its role in influenza A virus replication through siRNA knockdown, RNA extraction, RTŸqPCR, and plaque assays, revealing significant differences in viral replication between ÃÅ and ÅÉ hours postŸinfection. The study concludes that, although the PAŸhost protein P interaction was not conclusively established, the absence of host protein P appears to enhance viral replication, suggesting potential therapeutic strategies targeting proline metabolism. Limitations include the need for further repetition and the unknown mechanisms by which host protein P reduces viral replication, emphasising the complexity of hostŸvirus interactions and the importance of advancing fundamental understanding in this field.

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Availability

Detail Information

Series Title

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Call Number

EP BM-011

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

EP BM-011

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

RNA
Influenza A virus
proline
viral replication and proliferation
antiviral intervention
antiviral properties

Specific Detail Info

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Statement of Responsibility

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