Thesis

Molecular Surveillance Of Antimalarial Drug Resistance Genes In Plasmodium Falciparum Isolates In Indonesia

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Malaria is a disease caused by infection of the Plasmodium parasite that spreads through Anopheles
mosquitoes, which mainly plagues humans living in regions with poor healthcare and countries around
the equator. Among the five species capable of causing malaria in humans, Plasmodium falciparum
and Plasmodium vivax are noted as the most threatening species, as their manifestations in humans
are much stronger compared to the other species. Although various antimalarial treatments have been
given to stricken patients, cases of resistances to treatments rises in numerous places in the world.
The three most notable genes where the mutation related to drug resistance commonly occurs are the
PfCRT, PfMDR1, and PfKelch13. Existing diagnostic methods can only detect the presence of malaria
parasites in a contracted human, but not mutations related to drug resistance. Employing sample
amplification using PCR followed by Oxford Nanopore Technologies (ONT) sequencing utilizing the
Rapid Barcoding Kit (SQK-RBK110.96), this study seeks to develop and optimize a protocol to profile
genetic variances of Plasmodium falciparum collected from clinical patients in Papua, Indonesia.
Although optimization has yet to produce persistent results among trials, optimization attempts had
produced potential amplification configurations which can aid future studies to develop an established
protocol for amplification of the whole gene of interest aforementioned, including adjustments in
annealing temperature, extension duration, and extension temperature.

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Availability

Detail Information

Series Title

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Call Number

BM 24-005

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Malaria
Plasmodium falciparum
Drug Resistance
PCR amplification
ONT sequencing

Specific Detail Info

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Statement of Responsibility

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