Thesis

Assessing anti-influenza A response and host gene regulation of Daun Gatal (Laportea decumana)

---

Influenza A virus (IAV) remains a significant global health concern, prompting ongoing efforts to
explore novel antiviral agents. This study investigates the potential of Laportea decumana (Daun
Gatal) extract as an antiviral against IAV using A549 cell lines. The objective was to evaluate the
extract's impact on cell viability, cytotoxicity, and viral replication dynamics. A series of cytotoxicity
assays determined safe concentrations of the extract, followed by infection of A549 cells with IAV in
the presence of varying extract concentrations. Viral load was assessed using plaque assays and
quantitative PCR, while gene expression of IL-6 and the viral M gene was analyzed to understand the
extract's mechanism of action.

Findings reveal that Daun Gatal extract significantly reduces viral replication in a dose-dependent
manner, with the 100 μg/ml concentration showing the most pronounced effect. Moreover,
extract-treated cells exhibited reduced IL-6 expression, suggesting modulation of the inflammatory
response. However, cytotoxicity assays indicated concentration-dependent toxicity at higher doses.
In conclusion, Laportea decumana extract demonstrates promising antiviral activity against IAV by
inhibiting viral replication and modulating host immune responses. Further research is needed to
elucidate its specific mechanisms and potential application in combating influenza infections.

---

Availability

Detail Information

Series Title

-

Call Number

BM 24-022

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

-

Language

English

ISBN/ISSN

-

Classification

BM 24-022

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

Influenza A virus
antiviral activity
Laportea decumana extract
A549 cells
cytokine

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment