Books

Genomic Characterization of Pediococcus acidilactici through a Whole Genome Sequencing: Unveiling its Genetic Complexity and Biotechnological

---

Pediococcus is a genus that belongs to the family Lactobacillaceae. This family of bacteria consists of
gram-positive Lactic Acid Bacteria (LAB) commonly found in fermented products. Pediococcus
acidilactici holds an important role in food preservation due to its ability to inhibit food-borne
pathogens through pediocin production, a heat-stable antimicrobial peptide. While pediocin is the
main contributor to the antimicrobial activity of P. acidilactici there are other contributing factors
such as organic acid. The result of our previous study shows antimicrobial activity by P. acidilactici but
with the absence of pediocin detection. This result raises questions regarding the genetic factor of
antimicrobial activity of our P. acidialctici isolate. This study aimed to do bioinformatic analysis of
Whole Genome Sequence (WGS) of our P. acidilactici isolate. In particular to study genes that are
responsible for antimicrobial activity, particularly pediocin-producing genes. We hypothesized the
existence of pediocin-producing genes within the genome of our P. acidilactici isolates. Through a
comprehensive literature review and bioinformatic analysis, this study underlines the potential of our
P. acidilactici isolate as a probiotic for food fermentation and preservation. According to our analysis,
we found a significant hit of pediocin-producing genes by BLAST, however, the low similarity between
the search query and the sequence inside our genome assembly did not indicate the presence of
pediocin-producing genes. Additionally, we found a significant hit with a high degree of similarity of
lactic acid-producing genes inside our genome, which indicates the presence of at least one organic
acid inside our genome.

---

Availability

Detail Information

Series Title

-

Call Number

BI 24-001

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

-

Language

English

ISBN/ISSN

-

Classification

NONE

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

Genome
Pediococcus acidilactici
pediocin
whole genome sequence

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment