Thesis

Bioautography of Nudibranch (Phyllidia varicosa) Associated Microbe Ethyl Acetate Metabolite Extract as Antibacterial Agent

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Antimicrobial resistance is a threat that can be combated by discovering new antimicrobials. Previous
screening attempts by Badan Riset dan Inovasi Negara (BRIN)/ national research and innovation
agency resulted in the discovery of a mold specimen with a broad-spectrum antimicrobial activity.
Morphological observation of the specimen shows similarity to some species of mold belonging to
Aspergillus and Penicillium genera which both contribute to more than half of all marine fungal
isolate derived antibacterial. The mold itself is obtained during the screening process of Phyllidia
varicosa, a nudibranch species that is an understudied screening target for antibacterial compounds.
Additionally, there have been no previous studies conducted on the antibacterial effect of Phyllidia
varicosa or any nudibranch mold symbiont nor information of media type used to grown the mold
being able to induce antimicrobial production in mold sample, which becomes a point of novelty in
this research. It is the aim of this proposal to gather data from ethyl acetate extraction and
subsequent bioautography of the extract for identification of compound(s) responsible for eliciting
this antibacterial activity. This thesis finds that antimicrobial activity of the specimen is a polar
compound. However, the validity of the result is compromised with the coelution of several
compounds in the active fraction and it is recommended for future assays to separate the methods
used to fractionate the crude extract and antibacterial assay.

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Availability

Detail Information

Series Title

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Call Number

BM 24-002

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

antibacterial identification
marine antibacterial
ethyl acetate extraction
bioautography

Specific Detail Info

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Statement of Responsibility

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