Thesis

Investigation of Lemongrass Anti-Migration Properties Towards Non-Small Lung Cancer Cells

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Lung cancer, among all types of cancer, leads first in cancer incidence and accounts for the first largest
source of cancer-related mortality worldwide in 2022. Such a high incidence and mortality rate is
primarily due to the majority of patients being diagnosed with advanced stages of cancer.
Chemotherapy remains a primary option for metastasized NSCLC, however, chemotherapy still
possesses undesirable effects. Lemongrass is known to induce apoptosis in lung and cervical cancer.
However, one of its most abundant phytochemicals, citral, is suspected to have anti-migration
properties. Lemongrass is known to have different phytochemicals if it grows in different regions,
lemongrass extract in this study was first tested in cell viability assay. Following that, lemongrass
anti-cancer properties were assessed through gene expression analysis and scratch assay. Given it was
found that lemongrass extract is able to induce cell death, it was then continued to the next assay and
from gene expression analysis, it resulted in the downregulation of E-cadherin and downregulation of
vimentin. While the scratch assay showed that lemongrass extract is able to prevent cell migration.
This study found that lemongrass extract is able to induce cell death at various concentrations and it
has an anti-migratory effect on non-small cell lung cancer at the concentration of 0.25 μL/mL.
However, this study has some limitations, which are the use of a less precise cell viability assay and
low concentration of lemongrass extract, which can contribute to the ineffectiveness to upregulate
E-cadherin expression. Not only that, limitations from time and contamination resulted in some data
that could only be collected from a single duplicate. For future research, more biological replications
and use of the MTS assay can be used in future research

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Availability

Detail Information

Series Title

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Call Number

BM 24-005

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Migration
Lemongrass
Terpenoid
E-cadherin
Vimentin

Specific Detail Info

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Statement of Responsibility

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