Thesis

Detection and Genomic Characterisation of Dengue Virus (DENV) from Clinical Isolates

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Dengue virus (DENV) infection presents a significant global health threat, especially in Indonesia. This
study focuses on identification and genetic characterization of circulating DENV isolates in Timika and
Jambi through serotyping, genotyping, and mutation analysis to understand genetic diversity and
transmission dynamics. Samples from 119 and 115 suspected dengue patients in Timika and Jambi,
respectively, were tested for NS1 antigen and IgM/IgG antibodies. The extracted RNA underwent
qRT-PCR serotyping and whole genome sequencing for phylogenetic analysis. High seropositivity was
observed in Timika (90%), whereas the seropositivity in Jambi still needs further investigation. Both
regions were dominated by DENV-3. Interestingly, serotype shifting was observed in Jambi, which was
previously dominated by DENV-1. Phylogenetic analysis revealed the circulation of genotype I, IV, and
V (DENV-1), genotype Cosmopolitan (DENV-2), genotype I (DENV-3), and genotype II (DENV-4), all of
which clustered with isolates in surrounding regions. Future studies should therefore focus on these
genotypes, perform better surveillance for Timika patients, and perform ELISA IgG on Jambi samples
for accurate seroprevalence determination. Nevertheless, this study highlights the importance of
serotype identification, molecular surveillance, and seroprevalence determination, enhancing
knowledge of DENV transmission patterns.

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Availability

Detail Information

Series Title

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Call Number

BM 24-008

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Dengue virus
whole genome sequencing
phylogenetic analysis
genetic characterization
seroprevalence

Specific Detail Info

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Statement of Responsibility

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