Thesis

In-vitro Research for Anti-Adherence, Anti-Biofilm, and Anti-Inflammatory Properties of Thymus vulgaris Against Enterohemorrhagic Escherichia coli

---

Enterohemorrhagic Escherichia coli (EHEC) strains infect millions annually with their ability to colonize
the gut and form biofilms contributing to antibiotic resistance. Moreover, CXCL8 (IL-8), TNF-ɑ, and
ICAM-1 are genes involved in the inflammatory host response against EHEC infection. Thymus
vulgaris, generally known as common thyme, is a traditional medicinal plant with antibacterial
characteristics that contains a phenolic compound called thymol that can inhibit the growth of
various pathogenic bacteria. Therefore, this study aims to observe the presence of thymol in thyme
crude extracts and evaluate the cytotoxicity effects of thyme extracts towards HT29 cells, and
investigate the anti-adherence, anti-biofilm, and anti-inflammatory properties of thyme extracts
against EHEC as a potential treatment. Thyme crude extracts showed a total phenolic content of
54.13 ± 1.04 mg GAE/g DW. Furthermore, in vitro assessment of thyme extract towards HT29 cell
viability determined that at lower concentrations of below 500 μg/mL has no cytoxicity. Moreover,
thyme extracts at 400-25 μg/mL showed over 30-40% of anti-adherent and anti-biofilm properties,
while anti-inflammatory assessments found that thyme extract at 400 μg/mL was able to
downregulate CXCL8 (IL-8), TNF-ɑ, and ICAM-1 genes in the co-treatment and post-treatment groups,
but not in the pre-treatment group. This suggests that thyme can be used as an alternative herbal
medication but not as a preventative measure during EHEC infection. Future studies should optimize
anti-inflammatory assays using separate 24-well plates and repeating the assay for better results.

---

Availability

Detail Information

Series Title

-

Call Number

BM 24-009

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

-

Language

English

ISBN/ISSN

-

Classification

NONE

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

Enterohemorrhagic Escherichia coli
Thymus vulgaris
anti-adherence assay
anti-biofilm assay
anti-inflammatory assay

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment