Thesis

Single-cell Analysis of Long Non-coding RNAs (lncRNAs) in Tumor-Associated Macrophages (TAMs) in the Glioblastoma Microenvironment

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Glioblastoma (GBM) is the most severe type of brain tumor with poor prognosis and low survival rate,
characterized by molecular heterogeneity and immunosuppressive microenvironment which pose a
significant challenge in improving treatment strategies. It is found that the abundance of tumor-
associated macrophages (TAMs) in the GBM microenvironment contributes significantly to tumor

progression, yet the understanding of the underlying mechanism remains limited. Long non-coding
RNAs (lncRNAs) have emerged as key regulators in cancer biology, including their involvement in tumor
progression mechanisms mediated by TAMs. In this research, we performed single-cell RNA
sequencing analysis on 17,803 cells from 6 glioma patient samples to profile the GBM tumor
microenvironment landscape. We identified five TAM subtypes distinguished by unique gene
expression profiles, and highlighted the two primary TAM ontogenies: brain-resident microglia and

infiltrating monocyte-derived macrophages. Our analysis revealed PCED1B antisense RNA 1 (PCED1B-
AS1) as a significantly expressed lncRNA in TAMs, particularly associated with immune-related
pathways. The enrichment of PCED1B-AS1 showed a context-dependent impact on prognosis across
different TAM clusters, suggesting its role in regulating immune cell functions. We demonstrated that
PCED1B-AS1 potentially plays a crucial role in GBM microenvironment through its interaction with
TAMs. Our findings suggest that targeting PCED1B-AS1 could offer a novel therapeutic strategy for
GBM. The use of scRNA-seq was pivotal in uncovering the heterogeneity of TAMs and the specific roles
of lncRNAs within the tumor microenvironment.

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Availability

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Detail Information

Series Title

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Call Number

BI 24-007

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

glioblastoma
Single cell RNA Sequencing
PCED1B-AS1
long non-coding RNAs

Specific Detail Info

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Statement of Responsibility

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