Enrichment Program

Modeling Attachment Factor Used in Flaviviral Entry

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"Flavivirus is a genus of positive-sense single-stranded RNA viruses that are mostly transmitted
through mosquito or tick vectors and are known to be emerging and re-emerging viruses. Some of
the significant species that have been reported to cause an outbreak globally include DENV, YFV,
WNV, ZIKV and JEV. While some vaccines have been successful in preventing infections caused by
certain flaviviruses, specific treatments and vaccines for the majority of flaviviruses are yet to be
found. The early process of viral infection requires the binding of the viral surface protein, specifically
the envelope protein, to a host cell receptor. Although their mechanism of neuroinvasion is still
unknown, flaviviruses, including the aforementioned species, utilize the ubiquitous
Glycosaminoglycans (GAGs), particularly heparan sulfate, as attachment factors to bind to the host
cell. GAGs are long chains of polysaccharides composed of repeating units of sugar molecules, and
there are different types including heparan sulfate, heparin, chondroitin sulfate, dermatan sulfate,
and hyaluronic acid. Given the importance of GAGs in viral attachment, a comprehensive
understanding of the biology and pathogenesis of flavivirus is crucial in identifying potential
treatments. Hence, this study aims to investigate the role of heparin in flavivirus infection on SH-sy5y
cells through luciferase assay. The results showed that pre-treating the virus significantly inhibited
the infection on SH-sy5y cells. These findings can be beneficial for future references in the discovery
of treatments against flavivirus."

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Availability

Detail Information

Series Title

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Call Number

EP BM-041

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

heparin
Flaivivirus
Glycosaminoglycans (GAGs)
heparan sulfate
luciferase assay

Specific Detail Info

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Statement of Responsibility

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