Thesis
In Vitro Study of Sunscreen Product Towards UV-B and Protection Through Measurement of Cell Viability
Ultraviolet light is a type of light that is exhibited from the sun. Ultraviolet is divided into 3 types,
including UV-A, UV-B, and UV-C light. These Ultraviolet light might cause several adverse effects
including skin and eye damage. Hence, the application of sunscreen is important to prevent the
damage induced by UV exposure. Sunscreen is divided into several types including cream, stick,
spray, liquid, and etc. This study provides information about comparison of 2 types of commercial
sunscreen prototype, such as cream and stick sunscreen for its ability to protect against DNA damage
induced by UV-B exposure. Therefore, it is found that cream sunscreen (62% and 52%, respectively)
serves better protection against DNA damage and cell apoptosis than stick sunscreen (47.5% and
31%, respectively). However, the protection ability of sunscreen itself can be increased by adding
antioxidants or DNA repair enzymes towards the formulation. In addition, future research by
conducting the cytotoxicity study of the sunscreen prototypes and comparing other types of
sunscreen including liquid, gel, spray, etc can be conducted.
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