Thesis

The Effect of Blanching Pretreatment of Cabinet-Dried Javanese Turmeric (Curcuma xanthorrhiza Roxb.) on Its Cytoprotective and Hepatoprotective Activity In Vitro

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Hepatocellular carcinoma (HCC) is a primary liver cancer where its development and onset can be
caused by oxidative stress. Consequently, consuming Javanese Turmeric as an antioxidant can
compensate for the increase of oxidative stress and can be used as a potential liver cancer treatment.
However, during the manufacture of Javanese Turmeric as a herbal medicine or supplement, it can
alter the bioactive content and potentially trigger an oxidation reaction. Therefore, a pretreatment
known as blanching can prevent oxidation and reduce the loss of bioactive compounds in Javanese
Turmeric. This study aims to investigate the cytoprotective and hepatoprotective activity of blanched
cabinet-dried Javanese Turmeric extract (JTE) in an in vitro liver cancer model using CCl4
induced-HepG2 cell. The concentration of JTE at 1, 10, 100 μg/mL and CCl4 at 60 mM was determined
from the cytotoxicity assay. Following cytotoxicity assay, cytoprotective assay shows that blanched
cabinet-dried JTE were significantly protect the HepG2 cell towards CCl4. In the case of
hepatoprotective activity, blanched cabinet-dried JTE has a lower AST activity compared to
unblanched cabinet-dried JTE. These findings suggest that the blanching process might influence the
cytoprotective and hepatoprotective of cabinet-dried JTE. Future research should optimize the
blanching process and further examine oxidative stress markers, total antioxidant capacity (TAOxC),
and glutathione (GSH) assays to validate JTE's potency as a natural hepatoprotective agent.

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Availability

Detail Information

Series Title

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Call Number

PHA 24-014

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

blanching
Hepatocellular carcinoma
javanese turmeric
cabinet-dried
hepatoprotective

Specific Detail Info

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Statement of Responsibility

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