Thesis

The Effect of Pressure Blanching on the Hepatoprotective Activity of Sun-Dried Curcuma xanthorrhiza Roxb. in HepG2 Cells

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Liver cancer, primarily hepatocellular carcinoma (HCC), poses a major health burden globally due to
its high mortality rate. Reactive oxygen species (ROS), natural byproducts of liver metabolism, trigger
oxidative stress and contribute to HCC development. Curcuma xanthorrhiza Roxb. (CX), an Indonesian
native plant, offers numerous benefits including attenuating oxidative stress and protecting liver
function. However, CX’s bioactive compounds are prone to oxidation, reducing its therapeutic
potential. Pretreatment methods like pressure blanching can preserve these compounds by
inactivating oxidoreductase enzymes. Hence, this study investigates the hepatoprotective property of
pressure-blanched sun-dried CX against carbon tetrachloride (CCl4) in HepG2 cells. Cytotoxicity assays
using MTS determined safe concentrations of blanched (BL-CXE) and unblanched (UBL-CXE) CX
extract (CXE) and 50% cytotoxicity concentration (CTC50) of CCl4. The concentrations chosen and used
in subsequent experiments were 1, 10, and 100 μg/mL of CXE and 60 mM CCl4. Cytoprotective assay
showed all CXE concentrations protected cells from CCl4-induced damage. Aspartate
aminotransferase (AST) activity demonstrated an insignificant difference between the untreated cells
and CCl4-only cells, but BL-CXE consistently exhibited lower AST activity than UBL-CXE. These results
imply that pressure blanching may have an impact on the hepatoprotective property of CX. Future
research should optimize the pressure blanching method, the use of lower passage number, and
additional assessment of other liver injury biomarkers like alanine aminotransferase (ALT).
Assessment of glutathione and malondialdehyde (MDA) could also provide a comprehensive
understanding of the antioxidant activity of pressure-blanched CXE in mitigating oxidative stress and
lipid peroxidation.

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Availability

Detail Information

Series Title

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Call Number

PHA 24-006

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Antioxidant
hepatoprotective
Curcuma xanthorrhiza
pressure blanching
sun drying

Specific Detail Info

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Statement of Responsibility

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