Thesis
Spatial Distribution of Triterpenoid in Root and Leaves of Saga Plants (Abrus precatorius) Cultivated in Soil and Hydroponic Systems
The therapeutic benefits from the utilization of the roots and leaves saga plant have been known to
Asian cultures for centuries. Numerous studies have attributed the therapeutics properties to
triterpenoids, a class of secondary metabolites found in plants that are produced by the plant defense
system under stressful environmental conditions. Recent study in legume model plant, lotus
japonicus, reported increased accumulation via waterlogging stress, such as those from hydroponic
systems. This study aims to investigate the spatial distribution of triterpenoids in the roots and leaves
of other leguminous plant, saga, when cultivated in the similar hydroponic systems. Both descriptive
histochemical and quantitative UV-Vis analysis were employed in this study to obtain a deeper
understanding of triterpenoid distribution. The histochemical analysis found that a previously
expected structure called the secondary aerenchyma was absent in the roots of the saga plant. The
leaves of the saga plant were found to have unique terpenoid accumulating cellular structures that
are thought to be specialized cell tissues of the upper epidermis and the mesophyll of the leaf tissues.
The semi-quantitative analysis found that there is no significant difference in the distribution of
triterpenoid saponins between the roots of the plants grown in both mediums. In contrast, it was
found that there is a significantly higher distribution of triterpenoid saponins in the leaf tissues of the
hydroponics-grown saga plants in comparison to the leaf tissues of the soil-grown saga plants.
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