Thesis

Optimization Of Erythritol Production Using Molasses as A Potential Carbon Source

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Erythritol is a beneficial sugar alcohol that can be used as a sugar substitute for diabetic patients. It
has 0.2 kcal/g calories and still has 70-80% sweetness with the widely used sucrose. Erythritol
production through extraction and chemical synthesis is ineffective due to the small product yield,
making the commercial production expensive, so alternative synthesis using biotechnology is
important to explore. Previous research has shown that Moniliella pollinis SP5 was able to produce
erythritol using 200 g/L molasses as the carbon source. However, the glucose and sucrose become
very limited after four days that lead to cell death and halt erythritol production. Further optimization
is needed to produce a high erythritol yield with minimum cost. Erythritol is a bioproduct produced
by microorganisms as a response to high osmotic pressure and stress in the growth medium. High
concentrations of carbon source substrate can be applied in order to increase the osmotic pressure,
and it also acts as a bigger nutrient source for the yeast. Aside from that, an optimal carbon/nitrogen
(C/N) ratio can also cause the erythritol conversion pathway to be more favorable. Therefore, the
research aims to determine the optimal carbon source concentration, nitrogen source concentration,
and the carbon-to-nitrogen ratio that produce the highest erythritol productivity. The research also
aims to compare batch and fed-batch systems that are able to produce higher erythritol yield. The
experiment was conducted using different molasses concentrations as carbon source and yeast
extract as nitrogen source. Results showed the optimal carbon and nitrogen source was the media
combination of 200 g/L molasses and 1 g/L yeast extract that produced 13.929 g/L erythritol.
Moreover, fed-batch fermentation was able to increase erythritol production 1.15 times from batch
fermentation result.

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Availability

Detail Information

Series Title

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Call Number

BT 24-003

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Erythritol
yeast extract
Moniliella pollinis
Molasses
Fed-batch

Specific Detail Info

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Statement of Responsibility

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