Thesis

Characterization of Komagataeibacter intermedius postbiotics collected from bacterial cellulose fermentation in Hestrin-Schramm media and sugarcane molasses media

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Bacterial cellulose (BC) is a biopolymer currently being coveted for its versatility, sustainable sourcing
and biodegradability. The Indonesia International Institute for Life Sciences (i3L) lab has been
conducting research on BC fermentation and surrounding BC produced by the kombucha isolate
Komagataeibacter intermedius, with past findings showing a great prospect of the bacteria being able
to produce BC using not only commercial Hestrin-Schramm (HS) media, but also waste sugarcane
molasses media. However, the process has left behind waste in the form of spent media, which could
have been processed into cell-free postbiotics containing possibly beneficial metabolites produced by
K. intermedius during fermentation. This study aims to look into the potential antimicrobial activity
and acid content of the postbiotics to increase the efficiency of the lab’s BC production process.
Findings of this study discovered that K. intermedius postbiotics collected after 7-day BC fermentation
in sugarcane molasses media at 30°C was able to inhibit the growth of EHEC, L. monocytogenes, S.
typhimurium, and S. aureus. The postbiotics were found to have an acetic acid content of 2.41 g/L and
a gluconic acid content of 17.46 g/L, with no glucuronic acid. Meanwhile, K. intermedius postbiotics
collected from 3-day HS media fermentation and 14-day molasses media fermentation did not exhibit
promising antimicrobial activity despite containing acids. The author suggests further studies on the
mechanism of antimicrobial activity to better affirm the role of the acids, as well as looking into other
possibilities of characterization or applications of the postbiotics.

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Availability

Detail Information

Series Title

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Call Number

BT 24-004

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

komagataeibacter intermedius
Antimicrobial Activity
postbiotics
Characterization

Specific Detail Info

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Statement of Responsibility

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