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Thesis

Characterization of Komagataeibacter intermedius postbiotics collected from bacterial cellulose fermentation in Hestrin-Schramm media and sugarcane molasses media

Eileen Wijaya - Personal Name;

Bacterial cellulose (BC) is a biopolymer currently being coveted for its versatility, sustainable sourcing
and biodegradability. The Indonesia International Institute for Life Sciences (i3L) lab has been
conducting research on BC fermentation and surrounding BC produced by the kombucha isolate
Komagataeibacter intermedius, with past findings showing a great prospect of the bacteria being able
to produce BC using not only commercial Hestrin-Schramm (HS) media, but also waste sugarcane
molasses media. However, the process has left behind waste in the form of spent media, which could
have been processed into cell-free postbiotics containing possibly beneficial metabolites produced by
K. intermedius during fermentation. This study aims to look into the potential antimicrobial activity
and acid content of the postbiotics to increase the efficiency of the lab’s BC production process.
Findings of this study discovered that K. intermedius postbiotics collected after 7-day BC fermentation
in sugarcane molasses media at 30°C was able to inhibit the growth of EHEC, L. monocytogenes, S.
typhimurium, and S. aureus. The postbiotics were found to have an acetic acid content of 2.41 g/L and
a gluconic acid content of 17.46 g/L, with no glucuronic acid. Meanwhile, K. intermedius postbiotics
collected from 3-day HS media fermentation and 14-day molasses media fermentation did not exhibit
promising antimicrobial activity despite containing acids. The author suggests further studies on the
mechanism of antimicrobial activity to better affirm the role of the acids, as well as looking into other
possibilities of characterization or applications of the postbiotics.


Availability
#
Reference Collection (BT Thesis) BT 24-004
T202409064
Available
Detail Information
Series Title
-
Call Number
BT 24-004
Publisher
i3L, Jakarta : i3L, Jakarta., 2024
Collation
-
Language
English
ISBN/ISSN
-
Classification
NONE
Content Type
-
Media Type
-
Carrier Type
-
Edition
-
Subject(s)
komagataeibacter intermedius
Antimicrobial Activity
postbiotics
Characterization
Specific Detail Info
-
Statement of Responsibility
-
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No other version available

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Indonesia International Institute for Life Sciences - Learning Resources Center
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i3L Learning Resources Center (LRC) is vital part of your academic experience at Indonesia International Institute for Life-Sciences. LRC exists to support the teaching, learning and research programs of the Institute through the provision of high quality services and facilities which include access to a range of printed and digital resources primarily in the field of life-sciences and business. 

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