Thesis

Investigation of Ethyl-methanesulfonate (EMS) Treatment as A Mutagenesis Approach Toward the Enhancement of the Production of Carotenoids in Chlorella vulgaris

---

As the human population grows, the demand for organoleptic properties in food has linearly
increased, including the use of harmful synthetic colorant. Therefore, carotenoids, as a secondary
metabolites and natural compounds, can be used as an alternative to natural food preservatives.
They are also attributed to antioxidant properties which are beneficial for human consumption and
health. This research aims to find the optimum EMS concentration as a mutation agent for Chlorella
vulgaris, a microalgae species that are able to accumulate high carotenoids content. To further
improve the carotenoid content, chemical mutagenesis was used to create random modifications in
the algal base pair, specifically ethyl-methanesulfonate (EMS). An axenic single colony C. vulgaris
obtained was upscaled for the EMS treatment, and 57% cell viability was the lowest percent cell
viability obtained, treated with 200 mM EMS. However, there was an insignificant difference of
carotenoid accumulation in C. vulgaris treated with 200 mM EMS compared to untreated C. vulgaris.
The result suggests that the insignificant effect might be due to the percentage cell viability selected
that were still in the sublethal concentration of the EMS, which does not totally induce the genetic
variations, compared to the favored cell viability of 5-10%. Furthermore, the comparison of the
carotenoid content through the extraction process and analysis should be considered to validate the
current result, and lower cell viability should be further selected and investigated.

---

Availability

Detail Information

Series Title

-

Call Number

BT 24-016

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

-

Language

English

ISBN/ISSN

-

Classification

NONE

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

cell viability
Food preservatives
carotenoid
Chlorella vulgaris
EMS mutation

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment