Thesis

Development of Pediococcus acidilactici postbiotic loaded Biocellulose for Wound Healing Application

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Wound dressings play a crucial role in promoting efficient wound closure and healing by providing an
environment conducive to tissue repair. Traditional wound dressings, such as cellulose-based gauze,
often exhibit challenges in adhesion and exudate absorption, necessitating frequent replacements that
may cause additional trauma. Biocellulose (BC), derived from bacteria, presents a natural counterpart
with remarkable suitability as a wound dressing, yet lacks antimicrobial properties to prevent infection
from skin pathogens such as the Gram-positive bacteria Staphylococcus aurues and the Gram-negative
Pseudomonas aeruginosa. Antimicrobial postbiotics of lactic acid bacteria (LAB) is an alternative to
antibiotics as it prevents the emergence of antibiotic resistant bacteria and lactic acid in it has been
found to be the prominent contributor to the antimicrobial activity of LAB postbiotic. This study aims
to incorporate BC with postbiotic from Pediococcus acidilactici to equip it with antimicrobial
properties. The results showed success in producing an antimicrobial BC due to loading with postbiotic.
The maximum loading concentration of redissolved spray-dried postbiotic that showed maximum
inhibition based on agar diffusion method was 0.5 g/mL for S. aureus (ZOI = 35.27 mm) and 0.75 g/mL
for P. aeruginosa (ZOI = 26.27 mm). P. aeruginosa was more susceptible to the postbiotic due to
difference its structure and other unexplored. The loading efficiency of lactic acid into the BC was also
found to increase as the concentration of postbiotic increases. The loaded BC released a maximum
amount of lactic acid within the first 2 to 5 hours. The incorporation of BC was concluded to
successfully produce an antimicrobial BC, yet its releasing behavior remains to be inconclusive due to
lack of

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Availability

Detail Information

Series Title

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Call Number

BT 24-020

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Antimicrobial Activity
wound dressing
biocellulose
Pediococcus acidilactici
postbiotic
spray-drying

Specific Detail Info

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Statement of Responsibility

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