Thesis

Characterization of Potential Plastic Degrading Bacterial Isolates for Lipase Production

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Overproduction of plastics and difficulty to degrade the plastic have become one of the
major threats in the environmental sector. This plastic has been reported to accumulate in many
different places. Many methods have been used to solve the problem but resulting in other harmful
effects toward the environment. Methods by utilizing the enzyme from microbes can be feasible to
perform without additional disadvantages to the environment. Among the types of plastic, PET and
PU are the plastics which can be degraded by lipase enzymes due to the existence of ester bonds in
PET and PU. By using a hydrolysis mechanism, lipase enzymes are capable of cleaving the ester bond
present in those plastics resulting in an initial components structure of plastics. In the previous study,
several bacteria from Bantar Gebang and Cipayung have been reported to be able to degrade plastic.
However, the molecular mechanisms of those bacteria are still unknown. Hence, in this project, we
want to screen the potential plastic-degrading bacterial isolates for lipase production by performing
agar screening using olive oil and phenol red alongside with the enzyme activity assay using
nitrophenyl acetate. From these results, only three sample indicate consistency in both assay which
are Leachate_PET_BantarGebang, Leachate_PU_Cipayung, and Plastic_PU_Cipayung with
Leachate_PU_Cipayung sample indicate highest lipase activity on both assay. The sample of
Leachate_PU_Cipayung is also considered as a gram positive bacteria. Further investigations on
species identification are recommended by using specific methodology of 16s rRNA sequencing
technology for better identification.

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Availability

Detail Information

Series Title

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Call Number

BT 24-025

Publisher

i3L, Jakarta : i3L, Jakarta., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

plastics
bacteria
Lipase
Enzyme
Ester Bonds

Specific Detail Info

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Statement of Responsibility

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Other version/related

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