Thesis

Development Of Bacterial Cellulose Loaded with Pediococcus acidilactici Postbiotics as an Antimicrobial Food Packaging

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Bacterial cellulose (BC) is a promising packing material as it is biodegradable, non-toxic, has good
mechanical properties, and can form bonds with foreign particles. Meanwhile, P. acidilactici
postbiotics (metabolites) have the potential to be a natural food preservative as they contain
antimicrobial compounds that are heat- and pH-stable. This research aims to develop a novel
antimicrobial food packaging by loading P. acidilactici postbiotics into BC. The loading time of
postbiotics into BC and the drying time of postbiotics-loaded BC were optimized. The antimicrobial
activity of the postbiotics-loaded BC against Enterohaemorrhagic Escherichia coli (EHEC), Salmonella
typhimurium, Staphylococcus aureus, and Listeria monocytogenes was also evaluated on chicken
breast. BC had to be immersed in postbiotics for 8 h and dried at 60°C for 3 h to transform it into an
antimicrobial food packaging. Our postbiotics-loaded BC managed to reduce EHEC, S. typhimurium,
and S. aureus counts by ~1-1.5 log CFU/g, ~1.5-2.5 log CFU/g, and ~1 log CFU/g, respectively, compared
to the controls after storage at 4°C for 15 days, suggesting the potential of our postbiotics-loaded BC
to enhance the safety and extend the shelf life of chicken breast. However, our postbiotics-loaded BC
changed the pH, texture, and color of the chicken due to the high lactic acid content in the postbiotics.
Recommendations include finding an optimal dilution of P. acidilactici postbiotics to be loaded into BC
that could still exhibit antimicrobial activity without changing the organoleptic properties of the
chicken breast and testing the postbiotics-loaded BC on L. monocytogenes in chicken breast.

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Availability

Detail Information

Series Title

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Call Number

BT 24-009

Publisher

i3L, Jakarta : i3L Press., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

bacterial cellulose
Active packaging
postbiotics
antimicrobial packaging
Pediococcus acidilactic

Specific Detail Info

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Statement of Responsibility

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