Thesis

Extraction Optimization of Gotu Kola (Centella asiatica) Leaf using Ultrasound-Assisted Extraction

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The food technology industry is increasingly interested in innovative ways to include nutritious
ingredients that promote health in food products. The leafy green plant that we used as a traditional
medicine known as Gotu Kola (Centella asiatica), which is regional to tropical and subtropical regions of
Asia, has drawn a lot of interest from the food technology sector due to its potential to change functional
foods. Gotu Kola leaves are an interesting component because of their quantity of bioactive chemicals,
particularly Triterpenes like asiatic acid, asiaticoside, madecassic acid, and madecassoside. This project
focuses on the extraction of Gotu Kola leaves using ultrasound-assisted extraction and analysis to
determine the antioxidant properties of Gotu Kola leaf. By measuring the total phenolic content , total
flavonoid content, and antioxidant activity, the current research aims to evaluate the effectiveness of
extraction parameters such as ethanol %, temperature, and time of the Gotu Kola leaf. The Box-Behnken
design with 17 test runs (five center points) generated by RSM was utilized to optimize the parameters
needed for ultrasonic-assisted extraction in gotu kola leaf to increase operational efficiency. The
antioxidant activities, measured by DPPH and FRAP assays, also showed significant responses to
increasing temperature and ethanol concentration, with FRAP displaying a better model fit than DPPH.
The use of RSM in this study has successfully identified the optimal conditions for the
ultrasound-assisted extraction of bioactive compounds from Gotu Kola. The Response Surface
Methodology (RSM) recommends 20 minutes, 30°C, and 50% ethanol concentration for maximizing
extraction efficiency and antioxidant activity.

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Availability

Detail Information

Series Title

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Call Number

FT 24-036

Publisher

i3L, Jakarta : i3L Press., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Antioxidant activity
Ultrasound-assisted extraction
C. asiatica extract
Optimization
Response Surface Methodology, Ultrasound-Assisted

Specific Detail Info

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Statement of Responsibility

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