Thesis

In vitro Investigation on The Anti-adherence and Antibacterial Effect of Lactic Acid Bacteria Isolated from Cincalok on Listeria monocytogenes

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Humans have been co-existing with a whole population of microbes, where trillions of different
microbes are living inside the human GI tracts as a commensal gut microbiota. This condition
provides a mutual relationship where the gut microbiota serves as a natural protection for the
intestinal tract homeostasis. Nevertheless, the human intestinal tract is constantly exposed to
environmental microorganisms through foods, which greatly affects the gut integrity. Probiotics, such
as LAB, that are commonly contained in cincalok provide benefits for food preservation and intestinal
health. On the other hand, estimation of 600 million cases of foodborne diseases around the world
have been reported, where notable pathogens, such as Listeria monocytogenes, are responsible for
the increasing cases of foodborne illnesses due to unsafe foods. Considering the extensive benefits of
LAB, this research investigated the microbe-host interactions, antibacterial and anti-adherence
properties of LAB isolated from cincalok against Listeria monocytogenes in HT29 cells through an in
vitro study of gene analysis using qRT-PCR, broth microdilution and anti-adherence assay. The study
found that LAB isolated from cincalok possessed an anti-adherence property against L.
monocytogenes. However, due to a number of limitations, it is suggested that future research is
implemented to further understand the mechanisms of antimicrobial properties of LAB and explore
the effect of different MOIs with identified LAB isolates.

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Availability

Detail Information

Series Title

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Call Number

BM 24-042

Publisher

i3L, Jakarta : i3L Press., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Antimicrobial
Lactic Acid Bacteria
Foodborne pathogens
Listeria monocytogenes

Specific Detail Info

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Statement of Responsibility

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