Thesis

"Construction of Mini-genome System for both Rabies virus Laboratory Strain and Field Epidemic Strain

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Rabies virus (RABV) causes a zoonotic disease known as rabies that affects humans and mammals.
With a genome length of 12 kb, RABV is classified as a non-segmented, negative-stranded RNA virus
that encodes five different structural proteins. The nucleoprotein (N), phosphoprotein (P), and large
RNA polymerase protein (L) forms ribonucleoprotein (RNP); together with the viral genome it serves
as a transcription and replication template of the viral genome. This research aims to construct a mini-
genome system for the RABV laboratory strain CVS and the field epidemic strain 5989 to study its viral
replication and transcription mechanism. The result of luciferase-based assay showed that CVS strain
has an optimum ratio for mini-genome of 0.16 ng MG : 0.16 ng N : 0.04 ng P : 0.12 ng L as reflected on
the high luciferase activity (1.74 x 107 RLU/sec). While, two possible mini-genome ratios were found
for 5989 strain including 0.16 ng MG : 0.16 ng N : 0.04 ng P : 0.08 ng L or 0.16 ng MG : 0.16 ng N : 0.04
ng P : 0.12 ng L; however, no statistically significant differences was observed between the two ratios.
Though improvements still need to be applied for future studies, it can be concluded that the mini-
genome system was able to represent the genome replication and transcription activity of both RABV
strains through luciferase activity, which can be a stepping stone in developing therapeutic drugs or
vaccines.

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Availability

Detail Information

Series Title

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Call Number

BM 24-011

Publisher

i3L, Jakarta : i3L Press., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Rabies Lyssavirus
replication
transcription
mini-genome system
luciferase-based assay

Specific Detail Info

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Statement of Responsibility

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