Thesis

Investigation of the Antifungal and Anti-inflammatory Properties of Alpinian galanga Towards Epidermal Candida albicans Infection in HaCaT Cells

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Candida albicans is a yeast that resides on the human skin, however it can become an opportunistic
pathogen when the immune system is compromised or there are imbalances in the
microenvironment. This microorganism can cause candidiasis which is able to cause high mortality
rates in especially immunocompromised patients. Treating this infection is highly difficult due to
similarities in structure between the host and fungi as well as resistance to major antifungal drugs
that continues to rise. Plants are often utilized as effective and applicable alternative medicine,
including in fungal infection. Alpinia galanga is a rhizome with flavonoids that are antifungal and
anti-inflammatory in nature, thus have potential as treatment of candidiasis. However, in previous
studies difficulties in elucidating its active compounds led to unsuccessful results. This research aims
to perform methanolic extraction of Alpinia galanga, assess its antifungal activity, cytotoxicity in
epithelial or HaCaT cells, and determine its bioactive compounds. The assessment of antifungal
properties done via disc diffusion, well diffusion, and MIC determination showed that Alpinia galanga
did not exhibit any antifungal effect at concentrations from 62.5 μg/mL to 250 mg/mL. Furthermore,
the extract obtained showed cytotoxic activity that caused more than 50% reduction of cell viability
from concentrations of 12.5 mg/mL to 250 mg/mL, which is thought to be due to A. galanga causing
DNA damage and cell death through increasing ROS levels. Methanolic extraction of A.galanga was
able to yield alkaloids, terpenoids, and phenolics within the extract. The absence of flavonoids and
saponins may contribute to the lack of antifungal activity as they are the main contributors of the
antifungal nature of A. galanga. The phenolics and terpenoids obtained may cause cytotoxicity
towards HaCaT cells. Anti-inflammatory properties of A. galanga were unable to be determined due
to the lack of antifungal activity and the cytotoxic nature of the extract.

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Availability

Detail Information

Series Title

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Call Number

BM 24-017

Publisher

i3L, Jakarta : i3L Press., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Cytotoxicity
Candida albicans
Alpinia galanga
Antifungal
MIC
disc diffusion
well diffusion
Methanol extraction

Specific Detail Info

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Statement of Responsibility

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