Thesis

Investigation of the kunyit asam herbal drink potential as an alternative treatment for cisplatin resistance non-small cell lung cancer (NSCLC)

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Non-small cell lung cancer (NSCLC), accounting for more than 85% of lung cancer diagnoses, has
remained the leading cause of cancer-related death. Chemotherapy has been used as the gold
standard treatment to treat it, and one of the common drugs is cisplatin. However, cisplatin can lead
to resistance, resulting from a complex interaction of molecular pathways that reduces the efficacy of
cisplatin-based therapies, including the upregulated B-cell lymphoma 2 (BCL-2) gene.
Cisplatin-resistant NSCLC cells may have cross-resistance to other platinum-based medicines and
chemotherapeutic treatments that use similar pathways. This cross-resistance narrows the spectrum
of feasible treatment choices, underscoring the need for medicines that target different pathways or
processes unaffected by existing resistance mechanisms. Based on the well-documented anti-cancer
properties of curcumin and tamarind, the traditional Indonesian kunyit asam herbal drink provides a
culturally inherited and easily accessible mode of consumption, making it a promising avenue for
incorporating these bioactive compounds into cancer prevention and adjuvant therapy strategies.
This study aims to understand the efficacy of kunyit asam herbal drink as an alternative treatment for
cisplatin-resistant lung cancer, especially NSCLC. In this study, the result was found by using several
methodologies such as total phenolic acid quantification, cell viability assay, and gene expression
analysis. According to the findings, cisplatin-resistant cells had much greater viability rates after
cisplatin treatment, most likely due to improved DNA repair capacities and changed drug transport
routes. Notably, the kunyit asam extract exhibited dose-dependent cytotoxicity in both cell lines, with
substantial effects against cisplatin-resistant cells, indicating its potential as an alternative treatment
agent for chemoresistance. The extract's capacity to cause cell death via methods different than
standard chemotherapies highlights its potential as an adjuvant therapy. Furthermore, quantitative
gene expression analysis demonstrated that cisplatin treatment increased BCL-2, an anti-apoptotic
protein, more than kunyit asam extract in both cell lines, with the cisplatin-resistant line showing
much greater upregulation.

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Availability

Detail Information

Series Title

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Call Number

BM 24-043

Publisher

i3L, Jakarta : i3L Press., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

bcl-2
NSCLC
Chemoresistance
Kunyit Asam Herbal Drink
A549 Cell Line

Specific Detail Info

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Statement of Responsibility

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