Thesis

In Vitro Assessment of The Antibiofilm Properties of Curcuma SP. Essential Oil on Lactobacillus Acidophilus

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Biofilm formation poses a significant health challenge, playing a role in the pathogenesis of
biofilm-associated diseases, including dental caries, a chronic infectious disease with widespread
prevalence that significantly impacts oral health. Lactobacillus acidophilus is one of the primary
cariogenic microorganisms associated with the progression of dental caries. In order to address this
issue, Curcuma sp. essential oil (EO) has emerged as an alternative to chemical agents, providing
fewer side effects. Curcuma sp. EO is known to contain various polyphenols, attributed to its
antibacterial and antibiofilm properties, making it a promising candidate, particularly in the fields of
dentistry. This study aims to assess the antibiofilm properties and efficacy of Curcuma sp. EO against
L. acidophilus in an in vitro setting involving phytochemical screening, Kirby-Bauer assay, MIC
determination, and antibiofilm assay. Among three EO samples, EO3 possessed the highest phenolics
and flavonoids, followed by EO2, and EO1. Both EO2 and EO3 showed similar antibacterial activity
with zone of inhibition (ZOI) ranging between 10.05 ± 0.64 mm and 10.45 ± 0.92 mm, whereas EO1
was found to be the least effective with lowest ZOI. Similarly, the MIC determination revealed that at
50 μg/mL, EO2 and EO3 nearly inhibited bacterial growth, unlike EO1. Furthermore, EO2 and EO3
generally exhibited stronger antibiofilm effects as the concentration increased. In contrast, EO1
overall showed an increase in biofilm formation at higher concentrations. Therefore, both EO2 and
EO3 can be utilized as potential antibiofilm agents, particularly against L. acidophilus.

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Availability

Detail Information

Series Title

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Call Number

BM 24-022

Publisher

i3L, Jakarta : i3L Press., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Lactobacillus acidophilus
dental caries
antibiofilm properties
Curcuma essential oil

Specific Detail Info

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Statement of Responsibility

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