Thesis

Validation of Biomarkers for the Aging of the Anopheles dirus Mosquito

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Malaria, a global health concern, recorded approximately ÃÅÂ million cases and ÇÃÈ,ÁÁÁ deaths in
ÃÁÃÁ, with Southeast Asia ranking second in malaria cases Understanding the age structure of
mosquito populations is crucial for comprehending malaria transmission dynamics and assessing the
impacts of environmental changes and control measures Combating malaria involves control
programs aimed at shifting the mosquito populations age structure towards younger mosquitoes
unable to transmit the disease Conventional methods, such as insecticidetreated bed nets, reduce
the number of malariacarrying mosquitoes and likely shorten their lifespan, collectively contributing
to malaria prevention and control This research focuses on validating gene markers for age and parity
status in Anopheles dirus, a malaria vector in Thailand Using laboratoryreared An. dirus mosquitoes,
the study identifies promising agegrading biomarkers through previously conducted RNA sequencing
and RTqPCR analysis, specifically Histone HÄ, Innexin, and LRRrepeat protein Histone HÄ shows the
highest rate of increase in expression and is crucial in chromatin structure and gene regulation
Innexin and LRRrepeat protein also exhibit agerelated expression changes, with Innexin associated
with reproductive system development and LRRrepeat protein playing a role in immune defence
against the malaria parasite These findings highlight the potential of these genes as biomarkers,
positioning them as valuable tools for mosquito age assessment and offering indirect insights into the
completion of the gonotrophic cycle The study underscores the necessity for optimising molecular
agegrading techniques and validating them across diverse mosquito populations and environmental
conditions to enhance malaria control efforts

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Availability

Detail Information

Series Title

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Call Number

BM 24-028

Publisher

i3L, Jakarta : i3L Press., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Malaria
Plasmodium falciparum
Anopheles dirus
Age
Gonotrophic cycle

Specific Detail Info

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Statement of Responsibility

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