Thesis

Total Phenolic Content Yield and Stability Of Vanilla Planifolia In Natural Deep Eutectic Solvent Using Ultrasonic Assisted Extraction

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Vanilla (Vanilla planifolia) is prized for its unique flavor, typically consumed as vanilla extract, which
requires substantial extraction time using volatile ethanol. Natural deep eutectic solvents (NADES),
abundant and non-volatile, have shown solvating ability for bioactive compounds. This study examines
the yield of phenolics from vanilla beans based on total phenolic content (TPC), using NADES in thermal
and ultrasonic-assisted extraction (UAE). The solvents used were 40% ethanol, an equimolar
combination of lactic acid-propylene glycol (LA-PG) NADES, and choline chloride-glycerol (ChoCl-Gly)
NADES. Solvents were measured for pH, followed by extraction under thermal and UAE methods, and
TPC assay quantified phenolics concentration. Ethanol had the highest pH (10.57 ±1.37), followed by
ChoCl-Gly (6.91 ±1.37), and LA-PG (1.45 ±1.62). Thermal extraction yielded the highest phenolics with
LA-PG (427.330 ±2.03 μg GAE/ml), followed by ChoCl-Gly (279.943 ±10.39 μg GAE/ml), and ethanol
(258.771 ±8.64 μg GAE/ml). In contrast, UAE gave ethanol the highest yield (894.205 ±6.91 μg GAE/ml),
followed by LA-PG (695.488 ±12.7 μg GAE/ml), and ChoCl-Gly (448.567 ±18.94 μg GAE/ml). Significant
differences were observed (p=0.001) between the TPC of NADES and ethanol, alongside between the
TPC of vanilla beans extracted with UAE and thermal extraction (p

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Availability

Detail Information

Series Title

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Call Number

FT 24-001

Publisher

i3L, Jakarta : i3L Press., 2024

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Total phenolic content
Natural Deep Eutectic Solvent
Thermal Extraction
Ultrasonication

Specific Detail Info

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Statement of Responsibility

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